A simple procedure to obtain a medium-size oligogalacturonic acids fraction from orange peel and apple pomace wastes

Pectin oligosaccharides, which can be obtained from fruit wastes, have proven their potential as plant immune-system elicitors. Although the precise size of active species is still under investigation, medium oligosaccharides been reported most active. Three defined oligogalacturonic acid (OGAs) mixtures were produced commercial pectin, orange peel and apple pomace residues. The methodology developed involves two sequential treatments followed by stepwise ethanol precipitation. Without need chromatographic separations, three different fractions obtained. analyzed high performance anion exchange chromatography (HPAEC) completely characterized mass spectrometry, showing that small size, large contained OGAs degree polymerization 3 to 9, 6 18, 16 55, respectively.